Laboratory«Microbial Collection»

(founded in 1995)

 

 

Laboratory staff:

 

 

Leading researcher PhD, Associate Professor Alena I. Ladutska (ORCID: 0009-0002-5041-3607)

Researcher Hanna V. Kanterova

Researcher MSc Hanna A. Bareika

Researcher MSc Aliaksandra D. Herasimovich (ORCID: 0009-0005-5736-8273)

Researcher MSc Karyna V. Kantor

Researcher Sviatlana Leanovich (ORCID: 0009-0001-9855-3492)

Researcher Palina I. Arlouskaya

Researcher Victoriya V. Savich (ORCID: 0009-0004-3781-462X)

Junior Researcher MSc Darya Guliayeva (ORCID: 0009-0001-1894-7775)

Junior Researcher MSc Maxim Sikolenko (ORCID: 0000-0003-3136-7182)

 

Principal research trends:

 

- study of microbiota biodiversity in different ecosystems;

- molecular-genetic identification of bacteria, bacteriophages, filamentous and yeast fungi, development of PCR detection methods for target groups of microorganisms;

- studies on physiology, biochemistry and genetics of microorganisms representing diverse taxonomic groups;

- selection of microbial strains that produce biologically active compounds;

- depositing and maintenance of microbial cultures valuable for different areas of microbiology and biotechnology in a specialized collection fund;

- depositing of microbial strains for storage and patent procedure;

- optimization of long-term preservation methods for microbial cultures in collection depository;

- classification of information on deposited cultures, setting up databases describing microbial collection stock, consulting on identification and preservation of microorganisms;

- provision of microbial cultures upon request of scientific institutions, universities, production enterprises of microbiological profile;

- evaluation of antimicrobial activity of substances and materials.

 

 

Major investigation results:

 

– the novel species of bacteria Roseateles amylovorans and novel genus of bacteriophages Petuglutavirus were described;

- taxonomic diversity, virulence and antibiotic resistance profiles of Aeromonas species causing fish diseases in Belarus were investigated, and a method of PCR diagnostics of fish bacterioses was developed (in collaboration with the Institute of Fishery);

- cultures of psychrophilic, thermophilic and halophilic bacteria from various natural biotopes in Belarus were isolated, and their physiological and biochemical properties and biotechnological potential were characterised, with a specialised collection of extremophilic microorganisms being created as a result;

- a simple, environmentally safe laboratory method for producing wound coatings based on porous chitosan materials with immobilised bacteriophages Staphylococcus phage BIM BV-116 G and Escherichia phage BIM BV-67 G was developed; the coatings obtained were characterised by biodegradability, high water-holding capacity and bactericidal action (in cooperation with the Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus);

- Belarusian collection of non-pathogenic microorganisms incorporates over 3,250 cultures representing diverse taxonomic groups;

- Belarusian collection of non-pathogenic microorganisms is rated as the Object of National asset of the Republic of Belarus and was formally registered as a member of World Federation of Culture Collections (WFCC);

- catalogues and databases of microbial cultures comprising entries of type, reference and industrial strains were published in Belarusian, Russian and English versions;

- information about the Belarusian collection of non-pathogenic microorganisms is available in the Global Electronic Catalogue of Microorganisms, the Global Registry of Scientific Collections, on the website List of Prokaryotic Names with Standing in Nomenclature;

- guidelines for depositing microbial strains at Belarusian collection of non-pathogenic microorganisms were formulated;

- procedures of low-temperature conservation of bacteria, bacteriophages, mycelial and yeast-like fungi were introduced. Optimal regimens of refrigerating-defrosting and desiccation of cell suspensions and bacterial concentrates were defined;

- molecular-genetic identification of microorganisms from different taxonomic groups is performed on a permanent basis.

 

Major developments:

 

Biopesticide Multiphage to control bacterial diseases of vegetables in open field (Specifications BY 100289066.110-2013; State registration № 06-0087)

 

Method for complex diagnostics of fish bacteriosis pathogens (methodical recommendations approved by the National Academy of Sciences of Belarus 11.12.2023) (jointly with the Institute of Fishery)

 

Selected publications:

2025

1. Savich V., Akhremchuk A., Herasimovich A., Leanovich S., Valentovich L., Sidarenka A. Isolation, characterization, and whole-genome analysis of the novel temperate bacteriophage Ph-p5 infecting Glutamicibacter halophytocola. – Archives of Virology. – 2025. – Vol.170, article number 46. – https://doi.org/10.1007/s00705-025-06225-5

2. Leanovich S., Maximiuk Y., Dzegtsiaryk S., Sidarenka A. Diversity, virulence factors and antibiotic resistance profiles of Aeromonas species isolated from farmed fish in Belarus. – The Microbe. – 2025. – Vol.7, article number 100397. – https://doi.org/10.1016/j.microb.2025.100397

 

2024

1. Gvozdev M.Y., Turomsha I.S., Savich V.V., Faletrov Y.V., Sidarenka A.V., Shkumatov V.M., Loginova N.V. Sterically hindered phenolic derivatives: effect on the production of Pseudomonas aeruginosa virulence factors, high-throughput virtual screening and ADME properties prediction. – Archives of Microbiology. – 2024. – Vol.206, article number 91. –https://doi.org/10.1007/s00203-023-03827-y

2. Sidarenka A., Kraskouski A., Savich V., Akhmedov O., Nikalaichuk V., Herasimovich A., Hileuskaya K., Kulikouskaya V. Design of Sponge-like Chitosan Wound Dressing with Immobilized Bacteriophages for Promoting Healing of Bacterially Infected Wounds. –  Journal of Polymers and the Environment. – 2024. – Vol. 32, P. 3893-3909. – https://doi.org/10.1007/s10924-024-03241-5

3. Herasimovich A., Akhremchuk A., Valentovich L., Sidarenka A. Whole genome analysis, thermal and UV-tolerance of Lactococcus phage BIM BV-114 isolated from cheese brine. – Research in Microbiology. – 2024. – Vol. 175, Issues 5-6, article number 104203. – https://doi.org/10.1016/j.resmic.2024.104203

4. Kraskouski A., Mashkin M., Kulikouskaya V., Savich V., Sidarenka A., Pinchuk S., Li R. Design of highly porous materials based on chitosan/pectin interpolyelectrolyte complex for wound healing application. – Advances in Polymer Technology. – 2024. – Article number 8747902. – https://doi.org/10.1155/2024/8747902

5. Nikalaichuk V.V., Hiatsevich K.V., Kulikouskaya V.I., Ladutska A.I.., Sidarenka A.V. Study of the interaction between biogenic alginate-Ag nanoparticles and enrofloxacin: combinatory antibacterial effect and nanocomposite formation. –  BioNanoScience. – 2024. – Vol. 14, P. 346–358 – https://doi.org/10.1007/s12668-023-01257-0

 

2023

1.Guliayeva D., Akhremchuk A., Sikolenko M., Evdokimova O., Valentovich L., Sidarenka A. Roseateles amylovorans sp. nov., isolated from freshwater. – International Journal of Systematic and Evolutionary Microbiology. – 2023. – Vol. 73, Issue 11. – https://doi.org/10.1099/ijsem.0.006133

2. Sidarenka A.V., Leanovich S.I. , Kalamiyets E.I. , Vieira D.E.L. , Cardoso J.P.V. , Salak A.N. Commercial synthetic hydrotalcite as an adsorbent nanomaterial for removal of bacteria from contaminated water. – Environmental Engineering Research. – 2023. – Vol.28, Issue 3, article number 220063. –  https://doi.org/10.4491/eer.2022.063 

3. Kulikouskaya V., Nikalaichuk V., Hileuskaya K., Ladutska A., Grigoryan K., Kozerozhets I., Hovsepyan V., Sargsyan M., Sidarenka A. Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout. – International Journal of Biological Macromolecules. – 2023. – Vol. 251, article number 126302. – https://doi.org/10.1016/j.ijbiomac.2023.126302

9. Guliayeva D., Sidarenka A., Valentovich L. Introducing the novel species of the genus Roseateles, isolated from freshwater. – FEMS2023 abstract book : abstracts of 10th Congress of European Microbiologists, Hamburg, 9–13 July 2023. – Abst. W52. – P. 705. 

 

2022

1. Akhremchuk K.V., Skapavets K.Y., Akhremchuk A.E., Kirsanava N.P., Sidarenka A.V., Valentovich L.N. Gut microbiome of healthy people and patients with hematological malignancies in Belarus. – Microbiology Independent Research Journal (MIR Journal). – 2022. – Vol. 9, Issue 1, P. 18-30. – https://doi.org/10.18527/2500-2236-2022-9-1-18-30

2. Kraskouski A., Hileuskaya K., Ladutska A., Kabanava V, Liubimau A., Novik G., Nhi T.T.Y., Agabekov V. Multifunctional biocompatible films based on pectin-Ag nanocomposites and PVA: Design, characterization and antimicrobial potential. – Journal of Applied Polymer Science. – 2022. – Vol. 139, Issue 42, article number e53023. – https://doi.org/10.1002/app.53023 

3. Kraskouski A., Hileuskaya K., Nikalaichuk V., Ladutska A., Kabanava V. , Wanzi Yao, Lijun You Chitosan-based Maillard self-reaction products: Formation, characterization, antioxidant and antimicrobial potential. – Carbohydrate Polymer Technologies and Applications. – 2022. – Vol. 4, article number 100257. – https://doi.org/10.1016/j.carpta.2022.100257 

 

2021

1. Smirnova M., Miamin U., Kohler A., Valentovich L., Akhremchuk A., Sidarenka A., Dolgikh A., Shapaval V. Isolation and characterization of fast-growing green snow bacteria from coastal East Antarctica. – Microbiology Open. – 2021. –  Vol. 10, Issue 1, article number e1152. – https://doi.org/10.1002/mbo3.1152 

2. Zdorovenko E.L., Kadykova A.A., Shashkov A.S., Kiseleva E.P., Savich V.V., Novik G.I. The O-specific polysaccharides structures of Pseudomonas strains isolated from the Ficus elastica. – Carbohydrate Research. –  2021. – Vol. 499, article number 108235. – https://doi.org/10.1016/j.carres.2021.108235