Microbial Collection Laboratory

Head of the laboratory:

Anastasiya V. Sidarenka

Ph.D. in biology, Associate Professor (Ass. Prof.)

Phone: +375 (17) 393-09-21; 399-43-71
E-mail: a_sidarenkambio.bas-net.by, collectionmbio.bas-net.by

The laboratory was founded in 1995.

Laboratory staff:

Leading researcher PhD Alena I. Ladutska;
Researcher Hanna V. Kanterova;
Researcher MSc Hanna A. Bareika;
Researcher MSc Alexandra D. Gerasimovich;
Researcher Victoriya V. Savich;
Junior Researcher Svetlana I. Leonovich;
Junior Researcher MSc Darya Guliayeva.

Laboratory is responsible for function of Belarusian Collection of Non-pathogenic Microorganisms

Principal research trends:

-   depositing and maintenance of microbial cultures valuable for different areas of microbiology and biotechnology in a specialized collection fund;
-   selection and genetic engineering of highly active strains to develop biopreparations of novel generation;
-   molecular-genetic identification of bacteria, bacteriophages, filamentous and yeast-like fungi, studies on physiology and genetics of microorganisms representing diverse taxonomic groups;
-   optimization of long-term preservation methods for microbial cultures in collection depository;
- classification of information on deposited cultures, setting up databases describing microbial collection stock, consulting on identification and preservation of microorganisms;
- depositing of microbial strains for storage and patent procedure;
- provision of microbial cultures upon request of scientific institutions, universities, production enterprises of microbiological profile.

Major investigation results:

- Belarusian collection of non-pathogenic microorganisms incorporates over 2500 cultures representing diverse taxonomic groups.
- Belarusian collection of non-pathogenic microorganisms is rated as the Object of National asset.
- The collection was formally registered as a member of World Federation of Culture Collections (WFCC).
- Catalogues and databases of microbial cultures comprising entries of type, reference and industrial strains were published in Russian and English versions.
- Guidelines for depositing microbial strains at Belarusian collection of non-pathogenic microorganisms were formulated.
- Procedures for washless method of low-temperature conservation of bacteria, bacteriophages, mycelial and yeast-like fungi were introduced. Optimal regimens of refrigerating-defrosting and desiccation of cell suspensions and bacterial concentrates were defined.
- Molecular-genetical identification of microorganisms from different taxonomic groups is performed on a permanent basis.

Major developments:

Biopesticide Multiphage to control bacterial diseases of vegetables
in open field.
Specifications BY 100289066.110-2013; State registration № 06-0087.

Representative publications

  1. Smirnova M., Miamin U., Kohler A., Valentovich L., Akhremchuk A., Sidarenka A., Dolgikh A., Shapaval V. Isolation and characterization of fast-growing green snow bacteria from coastal East Antarctica. Microbiology Open. 2021; 10: e1152. https://doi.org/10.1002/mbo3.1152.
  2. Zdorovenko EL, Kadykova AA, Shashkov AS, Kiseleva EP, Savich VV, Novik GI. The O-specific polysaccharides structures of Pseudomonas strains isolated from the Ficus elastica. Carbohydr. Res. 2021 Jan;499:108235. doi: 10.1016/j.carres.2021.108235.
  3. Novik, G. Beneficial microbiota. Probiotics and pharmaceutical products in functional nutrition and medicine / G. Novik, V. Savich // Microbes Infect. – 2020. – Vol. 22, No 1. – P. 8-18.
  4. Zdorovenko, E.L. O-specific polysaccharides structures of Pseudomonas strains isolated from the strawberry leaves / E.L. Zdorovenko, A.A. Kadykova, A.S. Shashkov, E.P. Kiseleva, V.V. Savich, G.I. Novik // Carbohydr Res. – 2020. – Vol. 489. – P. 107932.
  5. Herasimovich A.D. Bacteriophages of lactic acid bacteria Lactococcus lactis and their resistance to physico-chemical factors / A.D. Herasimovich, A.V. Sidarenka // Microbial Biotechnologies: fundamental and applied aspects: collection of sci. papers / Institute of Microbiology of NASB ; eds: E.I.Kalamiyets [et al.]. – Minsk: «Belaruskaya navuka», 2020. – Vol. 12. – P. 40-58. [in Russian]
  6. Ladutska, A.I.  Physiological-biochemical and molecular-genetic properties of thermophilic sporulating bacteria isolated from natural sources / A.I. Ladutska, D.E. Guliayeva, S.I. Leanovich, A.V. Sidarenka // Microbial Biotechnologies: fundamental and applied aspects: collection of sci. papers / Institute of Microbiology of NASB ; eds: E.I.Kalamiyets [et al.]. – Minsk: «Belaruskaya navuka», 2020. – Vol. 12 – P. 112-122. [in Russian]
  7. Bareika, H.A. Optimization of PCR-based technique for diagnostics of bacterial pathogens of cucumber and tomato / H.A. Bareika, D.S. Paturemsky, A.V. Evdokimova, L.N. Valentovich, M.A. Titok, E.I. Kalamiyets, A.V. Sidarenka // Microbial Biotechnologies: fundamental and applied aspects: collection of sci. papers / Institute of Microbiology of NASB ; eds: E.I.Kalamiyets [et al.]. – Minsk: «Belaruskaya navuka», 2020. – Vol. 12 – P.144-159. [in Russian]
  8. Akhremchuk, E.V. Comparative examination of metagenomic DNA extraction methods for analysis of gut microbiota / E. V. Akhremchuk, A. E. Akhremchuk, S. V. Buinitskaya, A.V.Sidarenka, L.N.Valentovich // // Microbial Biotechnologies: fundamental and applied aspects: collection of sci. papers / Institute of Microbiology of NASB ; eds: E.I.Kalamiyets [et al.]. – Minsk: «Belaruskaya navuka», 2020. – Vol. 12 – P.1339-354. [in Russian]
  9. Novik, G. Beneficial lactic acid bacteria. Use of lactic acid bacteria in production of probiotics / G. Novik, V. Savich // Pocket guide to bacterial infections / ed. K. Balamurugan. – CRC Press, 2019. – P. 225-278.
  10. Novik G. Biodiversity conservation of phages and microbial populations / G. Novik, V. Savich, E. Kiseleva // Microbial Resource Conservation, Soil Biology / eds. S. Sharma, A. Varma. – Vol. 54. – Springer International Publishing AG, Springer Nature Switzerland AG, Cham, Switzerland, 2018. – Chapter 10. – P. 261-301.
  11. Novik, G. Classification and identification of bacteriophage receptors / G. Novik, V. Savich, A. Ladutska // Understanding microbial pathogens: current knowledge and educational ideas on antimicrobial research / eds. E. Torres-Hergueta, A. Méndez-Vilas. – Formatex Research Center, 2018. – P. 108-120.
  12. Novik, G. Waste degradation and utilization by lactic acid bacteria: use of lactic acid bacteria in production of food additives, bioenergy and biogas / G. Novik, O. Meerovskaya, V. Savich // Food Additives, Chapter 5., ed. by Prof. D. N. Karunaratne, G. Pamunuwa, 2017.– Rijeka, Croatia: InTech, 2017. – P. 105–146.
  13. Novik, G. Bacteriophages of Pseudomonas bacteria: application in medicine and agriculture. / G. Novik, V. Savich // The Battle Against Microbial Pathogens: Basic Science, Technological Advances and Educational Programs. A. Méndez-Vilas (ed.) – Formatex Research Center. 2015/2016 – V.1 – p. 295–304.
  14. Novik, G. An insight into beneficial Pseudomonas bacteria / G. Novik, V. Savich, E. Kiseleva // Microbiology in Agriculture and Human Health, Chapter 5. // ed. by Dr. Mohammad Manjur Shah, 2015 –– Rijeka, Croatia: InTech, 2015. – P. 73–105.
  15. Probiotics / G. Novik [et al.] // Biotransformation of Waste Biomass into High Value Biochemicals / S. K. Brar (eds.) [et al.]. – New York: Springer Science+Business Media, 2014. – P. 187–235.

Государственное научное учреждение
Институт микробиологии Национальной академии наук Беларуси

Адрес: 220084, ул. Купревича, 2, Минск, Республика Беларусь
Приемная:
Тел./Факс: +(375) 17 395-47-66
E-mail: microbio@mbio.bas-net.by

Контактное лицо: ученый секретарь Левчук Ольга Дмитриевна

Тел.:+(375) 17 394-47-18
E-mail:scient_secretary@mbio.bas-net.by

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