Laboratory of Molecular Diagnostics and Biological Control of Phytopathogenic Microorganisms

Head of the laboratory:

Marina N. Mandryk-Litvinkovich

Ph. D. in biology, Associate Professor (Ass. Prof)

Phone: +375 17 396-86-03, +375 17 242-99-27
E-mail: biocontrol@mbio.bas-net.by

The laboratory was founded in 2018 after restructuring of the laboratory of biological control agents. In the year 2020 laboratories scientific focus was enriched by the biodeterioration research.

Principal research trends:

- diagnostics of fungal and bacterial crop pathogens using DNA typing procedure;
- isolation, selection and genetic engineering of microbial strains showing phytoprotecting and growth-stimulating properties, directed regulation of their antagonistic activity;
- elaboration of competitive technologies for production of biological control agents;

- assessment of fungal contamination in buildings, development of environmentally safe methods for protection against mould damage;
- testing resistance of different materials to mould attack in model experiment;
- studies on the diversity and biotechnological potential of microorganisms isolated from natural and anthropogenic substrates brought from Antarctica.

Major investigation results:

- collection of microbial cultures displaying high antagonistic activity toward plant pathogens was set up; mechanisms governing antimicrobial action of introduced cultures were deciphered; key factors determining synthesis of microbicidal metabolites by the studied strins were identified;
- biotechnologies were developed and pilot-plant production of microbial preparations for crop cultivation was arranged;

-      the main reasons and factors of mould growth in buildings and premises were revealed;
-      in collaboration with the Institute of Physics NAS of Belarus the approaches were developed for application of  low-temperature plasma technology to decontaminate materials infected with spores of mould fungi;
-      the Antarctic isolates were screened for psychrotolerant cultures with elevated antagonistic activity against plant pathogens.

Major developments:

- determinative guide of phytopathogenic microorganisms for rapid and precise molecular-genetic diagnosing of cultivar diseases;
- biopesticides Fruitin and Ecosad (to control fruit pathogens), Betaprotectin (to suppress clamp rot of sugar beet, root rots of tomatoes and cucumbers), Ecogreen (to counter infection of cucumbers and greens spices in small-scale hydroponic culture), Xantrel (to protect potatoes from pests and pathogens), Bactosol (to shield potato seedlings during vegetation season and tubers in the course of storage), complex microbial preparations Agrorevitol (to degrade residual herbicides in soil and to promote crop harvests) and Polybact (to restore soil microbial cenoses and to increase productivity of cultivans);

-      collection of fungi and DNA bank of micromycetes responsible for biodeterioration processes and engaged in fungal resistance laboratory trials were set up;
-      in cooperation with the researchers from Belarusian State Technological University the method of producing biopreparation “Phlebiopin” based on basidiomycete Phlebiopsis gigantea was elaborated and introduced into practice to protect conifer plantations from root rot.

Representative publications:

  1. Bacteria Bacillus amyloliquefaciens 355 possessing complex antimicrobial, growth-stimulating and cellulolytic activities / T. L. Nosonova, M. N. Mandryk-Litvinkovich, O. V. Molchan, E. I. Kolomiets // Microbial biotechnologies: basic and applied aspects: Annual Transact. Institute Microbiol. NAS of Belarus, eds. E. Kolomiets, A. Lobanok – Minsk, 2016. – Vol. 8. – P. 226-235.
  2. Molecular-genetical structural peculiarities of phage Pf-10 / T. A. Pilipchuk, L. N. Valentovich, M. A. Titok, E. I. Kolomiets // Doklady Nat. Acad. Nauk Belarusi. – 2017. – Vol. 61, № 1. – P. 78-84.
  3. Principles of biopesticide Ecogreen production technology / M. N. Mandryk-Litvinkovich, T. L. Nosonova, V. N. Kuptsov, G. K. Zhuromsky, E. G. Shinkorenko, E. I. Kolomiets // Microbial biotechnologies: basic and applied aspects: Annual Transact. Inst. Microbiol. NAS Belarus eds. E. I. Kolomiets, A. G. Lobanok. – Minsk, 2017. – Vol. 9. – P. 210-224.

Государственное научное учреждение
Институт микробиологии Национальной академии наук Беларуси

Адрес: 220141, ул. Купревича, 2, Минск, Республика Беларусь
Факс: +(375) 17 395-47-66
E-mail: microbio@mbio.bas-net.by
Контактное лицо:
ученый секретарь Демешко Ольга Дмитриевна


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