Laboratory “Center of analytical and genetic engineering research”

HEAD OF THE LABORATORY: 

Leonid N. Valentovich, Ph.D.

Phone: +375 17 267-41-44
Fax: +375 17 267-47-66
E-mail: valentovichmbio.bas-net.by

The Center was founded in 2009.

 

 Laboratory staff:

1) Olesya Evdokimova

2) Ekaterina Semenchukova

3) Lev Chebotarev

4) Anna Muratovа

5) Nadezhda Pilipenok

6) Svetlana Buinitskaya

7) Artur Akhremchuk

8) Katerina Akhremchuk

 

The main directions of research and prospects of scientific and technical activities:

- construction of genetically engineered strains producers of biologically active compounds;

- molecular genetic identification of microorganisms;

- sequencing the genomes of bacteria and viruses;

- molecular plant pathology;

- qualitative and quantitative analysis of organic compounds by gas-liquid chromatography and high performance liquid;

- spectrophotometry in the ultraviolet, visible and near-infrared (190-1100nm).

 

Laboratory equipment:

- high pressure liquid chromatograph 1260 Infinity with a mass spectrometer Agilent 6530 Accurate-Mass Q-TOF LC/MS System;

- high pressure liquid chromatograph Shimadzu LC-30 Nexera;

- gas chromatograph AgilentTechnologies 7890B with a flame ionization and phosphorus-nitrogen detector;

- headspace sampler Agilent 7697A;

- gas chromatograph Chrom 5 with a flame ionization detector;

- shaker incubator INFORS HT Ecotron

- CO2-incubator HeraCell 150;

- ultracentrifuge Beckman Coulter Allegra X-30R and Beckman Coulter Avanti J-30I with a set of rotors;

- gradient DNA thermocycler Agilent SureCycler 8800;

- gradient DNA thermocycler Bio-Rad T100;

- thermocycler with an optical module for real-time PCR Agilent Mx3005P;

- gel documentation system Bio-Rad Gel Doc MP;

- electroporator for transforming microorganisms Bio-Rad MicroPulser;

- ultra-low temperature freezer ilShin DF8520;

- chromatographic system Bio-Rad NGC Scout™ 10;

- nano-spectrophotometer Implen Pearl 330;

- spectrophotometer SP-830 Plus Metertech;

- PCR Workstation UVT-S-AR BioSan;

- DNA Analysis System Li-Cor 4300;

- class II Biological Safety Cabinet Esco AC2-5EI;

- system for vertical electrophoresis of proteins CS-300V and Clamp Complete Blotting System CVS10CBS Cleaver Scientific Ltd;

- Trans-Blot BioRad Turbo Transfer System;

- light microscope Olympus BX-43;

- analytical scales Sartorius CPA225D;

and a set of reagents and equipment to perform biochemical, microbiological, molecular biology and laboratory tasks.

Recent Publications:

  1. Yanina A. Delegan, Leonid N. Valentovich, Samira M. Shafieva, Khudaverdi G. Ganbarov, Andrey E. Filonov, Mikhail B. Vainstein. Characterization and genomic analysis of highly efficient thermotolerant oil-degrading bacterium Gordonia sp. 1D // Folia Microbiol (Praha). 2018 Jun 27. doi: 10.1007/s12223-018-0623-2. [Epub ahead of print]. P.1-8.
  2. Olesia V. Evdokimova, Vlad E. Miamin, Leonid N. Valentovich. Biochemical and molecular genetic characteristics of Bacillus pumilus bacteria isolated in Belarus // Journal of the Belarusian State University. Biology. – 2018. – №1. – P.38-49. (in Russian)
    (PDF, 970 Kb)
  3. Pilipchuk T. A., Valentovich L. N., Titok M. A., Kolomiets E. I. Peculiarities of molecular-genetic structure of phage Pf-10. Doklady Natsional’noi akademii nauk Belarusi [Doklady of the National Academy of Sciences of Belarus], 2017, vol. 61, no. 1, pp. 78–84. (in Russian)
  4. Mandryk-Litvinkovich, M.N., Muratova, A.A., Nosonova, T.L., Evdokimova O.V., Valentovich L.N., Titok, M.A., Kolomiets, E.I. Molecular genetic analysis of determinants defining synthesis of 2,4-diacetylphloroglucinol by Pseudomonas brassicacearum BIM B-446 bacteria // Applied Biochemistry and Microbiology (Moscow), 2017, Vol. 53, № 1, P. 38–46.
    (DOI: 10.7868/S0555109917010123)
  5. Muratova A.A., Mandryk-Litvinkovich M.N., Nasonova T.L., Valentovich L.N., Kolomiets E.I., Titok M.A. Molecular genetic analysis of the determinants associated of antimicrobial properties of bacteria Pseudomonas brassicaсearum BIM B-446 // Proceedings of the National Academy of Sciences of Belarus. Series of Biological Sciences. 2016. № 3. pp. 81-84. (in Russian)
  6. Ananyeva I.N., Rubel I.E., Valentovich L.N., Kolomiets E.I., Titok M.A. Molecular genetic and functional analysis of cry-genes from native strains of Bacillus thuringiensis // Proceedings of the National Academy of Sciences of Belarus. Series of Biological Sciences. 2015. № 2. pp. 50-55. (in Russian)
  7. Lagonenko A.L., Sadovskaya O., Valentovich L.N., Evtushenkov A.N. Characterization of a new ViI-like Erwinia amylovora bacteriophage phiEa2809 // FEMS Microbiol Lett. 2015 Apr;362(7). pii: fnv031. doi: 10.1093/femsle/fnv031.
  8. Nikolaichik Y, Gorshkov V, Gogolev Y, Valentovich L, Evtushenkov A. Genome Sequence of Pectobacterium atrosepticum Strain 21A // Genome Announc. 2014 Sep 18;2(5). pii: e00935-14. doi: 10.1128/genomeA.00935-14.
  9. Charniauskaya M.I., Kazlova M.V., Titok M.A. Metabolic potential of microorganisms isolated from contaminated soils // Vestnik BSU. Ser. 2. 2014. N 3. P. 33-37. (In Russian)
  10. Valentovich L.N., Laziuk S.K., Kolomiets E.I., Titok M.A. Molecular genetic identification of biotechnologically relevant bacteria of the genus Bacillus // Reports of the National Academy of Sciences of Belarus. – 2014. – Vol.58, №1. – P. 89-93. (in Russian)

Государственное научное учреждение
Институт микробиологии Национальной академии наук Беларуси

Адрес: 220141, ул. Купревича, 2, Минск, Республика Беларусь
Факс: +(375) 17 267-47-66
E-mail: microbio@mbio.bas-net.by
Контактное лицо:
ученый секретарь Сидоренко Анастасия Вячеславовна


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