Laboratory “Center of analytical and genetic engineering research”

Head of the Center:  Ph.D. Leonid N. Valentovich

Phone: +375 17 267-41-44

Fax: +375 17 267-47-66

E-mail: valentovichmbio.bas-net.by

 

The Center was founded in 2009.

 

 Laboratory staff:

1) Olesya Evdokimova

2) Ekaterina Semenchukova

3) Lev Chebotarev

4) Anna Muratovа

5) Nadezhda Pilipenok

6) Svetlana Tsynkevich

 

The main directions of research and prospects of scientific and technical activities:

- construction of genetically engineered strains producers of biologically active compounds;

- molecular genetic identification of microorganisms;

- sequencing the genomes of bacteria and viruses;

- molecular plant pathology;

- qualitative and quantitative analysis of organic compounds by gas-liquid chromatography and high performance liquid;

- spectrophotometry in the ultraviolet, visible and near-infrared (190-1100nm).

 

Laboratory equipment:

- high pressure liquid chromatograph 1260 Infinity with a mass spectrometer Agilent 6530 Accurate-Mass Q-TOF LC/MS System;

- high pressure liquid chromatograph Shimadzu LC-30 Nexera;

- gas chromatograph AgilentTechnologies 7890B with a flame ionization and phosphorus-nitrogen detector;

- gas chromatograph Chrom 5 with a flame ionization detector;

- shaker incubator INFORS HT Ecotron

- CO2-incubator HeraCell 150;

- ultracentrifuge Beckman Coulter Allegra X-30R and Beckman Coulter Avanti J-30I with a set of rotors;

- gradient DNA thermocycler Agilent SureCycler 8800;

- gradient DNA thermocycler Bio-Rad T100;

- thermocycler with an optical module for real-time PCR Agilent Mx3005P;

- gel documentation system Bio-Rad Gel Doc MP;

- electroporator for transforming microorganisms Bio-Rad MicroPulser;

- ultra-low temperature freezer ilShin DF8520;

- chromatographic system Bio-Rad NGC Scout™ 10;

- nano-spectrophotometer Implen Pearl 330;

- spectrophotometer SP-830 Plus Metertech;

- PCR Workstation UVT-S-AR BioSan;

- DNA Analysis System Li-Cor 4300;

- class II Biological Safety Cabinet Esco AC2-5EI;

- system for vertical electrophoresis of proteins CS-300V and Clamp Complete Blotting System CVS10CBS Cleaver Scientific Ltd;

- Trans-Blot BioRad Turbo Transfer System;

- light microscope Olympus BX-43;

- analytical scales Sartorius CPA225D;

and a set of reagents and equipment to perform biochemical, microbiological, molecular biology and laboratory tasks.

Recent Publications:

  1. Pilipchuk T. A., Valentovich L. N., Titok M. A., Kolomiets E. I. Peculiarities of molecular-genetic structure of phage Pf-10. Doklady Natsional’noi akademii nauk Belarusi [Doklady of the National Academy of Sciences of Belarus], 2017, vol. 61, no. 1, pp. 78–84. (in Russian)
  2. Muratova A.A., Mandryk-Litvinkovich M.N., Nasonova T.L., Valentovich L.N., Kolomiets E.I., Titok M.A. Molecular genetic analysis of the determinants associated of antimicrobial
    properties of bacteria Pseudomonas brassicaсearum BIM B-446 // Proceedings of the National Academy of Sciences of Belarus. Series of Biological Sciences. 2016. № 3. pp. 81-84. (in Russian)
  3. Ananyeva I.N., Rubel I.E., Valentovich L.N., Kolomiets E.I., Titok M.A. Molecular genetic and functional analysis of cry-genes from native strains of Bacillus thuringiensis // Proceedings of the National Academy of Sciences of Belarus. Series of Biological Sciences. 2015. № 2. pp. 50-55. (in Russian)
  4. Lagonenko A.L., Sadovskaya O., Valentovich L.N., Evtushenkov A.N. Characterization of a new ViI-like Erwinia amylovora bacteriophage phiEa2809 // FEMS Microbiol Lett. 2015 Apr;362(7). pii: fnv031. doi: 10.1093/femsle/fnv031.
  5. Nikolaichik Y, Gorshkov V, Gogolev Y, Valentovich L, Evtushenkov A. Genome Sequence of Pectobacterium atrosepticum Strain 21A // Genome Announc. 2014 Sep 18;2(5). pii: e00935-14. doi: 10.1128/genomeA.00935-14.
  6. Charniauskaya M.I., Kazlova M.V., Titok M.A. Metabolic potential of microorganisms isolated from contaminated soils // Vestnik BSU. Ser. 2. 2014. N 3. P. 33-37. (In Russian)
  7. Valentovich L.N., Laziuk S.K., Kolomiets E.I., Titok M.A. Molecular genetic identification of biotechnologically relevant bacteria of the genus Bacillus // Reports of the National Academy of Sciences of Belarus. – 2014. – Vol.58, №1. – P. 89-93. (in Russian)

Государственное научное учреждение
Институт микробиологии Национальной академии наук Беларуси

Адрес: 220141, ул. Купревича, 2, Минск, Республика Беларусь
Факс: +(375) 17 267-47-66
E-mail: microbio@mbio.bas-net.by
Контактное лицо:
ученый секретарь Сидоренко Анастасия Вячеславовна


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